This museum was founded in 1991 by Mr. Philip Demertzis-Bouboulis, Bouboulina’s fourth generation descendant, in his effort to save the mansion from collapse. It is a non-profit organisation and its main objective is to repair and maintain the historic museum's historic building, whilst at the same time to recount the story of the Greek War of Independence with emphasis on the life of Laskarina Bouboulina (Greek: Λασκαρίνα Μπουμπουλίνα), 11 May 1771 - 22 May 1825) who was one of the most important Greek heroines of the Greek War of Independence in 1821.

The museum's mansion was built by a Mauritanian architec, towards the end of the 17th century and has a Π shape. This shape, in Spetsiot architecture, was related to the importance of its owner. Most of the Spetsiot lords of the time had houses of this shape. The mansion consists of a ground floor with two floors above. An external stone staircase connects the front yard with the first floor, where the museum is housed. Visiting areas on the first floor consist of four large rooms.